Spinning disk confocal psf5/29/2023 Spinning Disk images are very well suited for deconvolution with the Spinning Disk Deconvolution Option, specially designed to take into account the optical properties of spinning disk microscopes such as the pinhole size and distance on the disk. For fixed samples, a point-scanning Confocal Microscope is, in some cases, a better solution.Ī Yokogawa Disk is a Nipkow disk with an array of microlenses in (or right after) the holes used in many commercial spinning disk microscopes. Spinning disk microscopes are very good for living samples because they are fast and have relatively low phototoxicity. Applied to a Fluorescence Microscope, it allows the sample to be scanned with an array of light points transmitted through these holes, participating in some of the advantages of a Confocal Microscope. Long lamp life (1500 hr) and low maintenance cost. Permits full spectrum (360 700 nm) confocal imaging. Allows long term recording of biological events. A Nipkow disk is a spinning disk with a series of equally distanced circular holes of equal diameter drilled in it. Binocular viewing of confocal image allows easy set up and color viewing. A Nipkow spinning disk microscope is one type of Fluorescence Microscope.
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